Selected Topics in the Analysis of Lipids

Review Articles from ‘Advances in Lipid Methodology—One and Two’

(by William W. Christie)

• Solid-phase Extraction Columns in the Analysis of Lipids
• Preparation of Ester Derivatives of Fatty Acids for Chromatographic Analysis
• Preparation of Lipid Extracts from Tissues
• The Chromatographic Resolution of Chiral Lipids
• Detectors for HPLC of Lipids with Special Reference to Evaporative Light-scattering Detection

General Methodology

• Why Methods go Wrong
• Laboratory Accreditation

Fatty Acid Analysis

• What Column do I Need for Gas Chromatographic Analysis of Fatty Acids?
• Fatty Acid Analysis by HPLC
• Alternatives to Methyl Esters for GC Analysis
• CLA—A Practical Guide (from INFORM) (by William W. Christie, Pierre Juaneda and Jean Louis Sébédio).
• Infrared Spectroscopy for Rapid Determination of Total Saturated, trans, Monounsaturated, and Polyunsaturated Fatty Acids (by Magdi M. Mossoba, Hormoz Azizian, and John K.G. Kramer)

Mass spectrometry

• Use of Lithiated Adducts for Structural Analysis of Acylglycerols by ESI-MS (by Jiann-Tsyh (Ken) Lin)
• Identification of FAME Double Bond Location by Covalent Adduct Chemical Ionization (CACI) Tandem Mass Spectrometry (by J. Thomas Brenna and Cynthia Tyburczy)

Other Chromatographic Methods

• The Use of Countercurrent Chromatography (CCC) in Lipid Analysis (by Walter Vetter)
• Gas Chromatographic Analysis of Plant Sterols (by Jill Winkler-Moser)
• Analysis of Tocopherols and Tocotrienols by HPLC (by Anna-Maija Lampi)
• Reversed-phase HPLC of Triacylglycerols
• Thin-layer Chromatography
• Structural Analysis of Triacylglycerols
• High-temperature Gas Chromatography of Triacylglycerols
• Modifed AOCS Method for Oil Content Determination (by Keshun Liu)
• Lipidomics—A Personal View (by W.W. Christie)