Feed Microscopy Short Course
Focusing upon Ruminant Feed & Restricted Use Proteins

Course Schedule and Outline

Thursday, December 9	8:00 a.m.-5:00 p.m.
Friday, December 10	8:00 a.m.-5:00 p.m.
Saturday, December 11	8:00 a.m.-4:00 p.m.

1. Introduction to Microscopy (Instructors: Patricia Ramsey, Neil Vary)
1. Equipment
1. Stereoscope
2. Compound Microscope
2. Principles of Ingredient Identification
1. Stereomicroscopy
2. Compound microscopy
3. Reference Ingredients Collection
3. Equipment and Reagents
4. Procedures for Analysis of Feed Ingredients
1. Qualitative
2. Quantitative
2. Feed Ingredients of Plant Origin
1. Corn (Instructor: Patricia Ramsey)
1. Whole
2. Ground
3. Gluten feed
4. Gluten meal
5. Distillers dried grains
2. Grain Sorghum (Instructor: Patricia Ramsey)
1. Whole
2. Ground
3. Oats (Instructor: Neil Vary)
1. Whole
2. Ground
4. Rice (Instructor: Patricia Ramsey)
1. Whole (rough or paddy)
2. Hulls
3. Bran
5. Barley (Instructor: Patricia Ramsey)
1. Whole
2. Ground
3. Brewers dried grains
6. Wheat (Instructor: Neil Vary)
1. Whole
2. Ground
3. Mill run
7. Unknown Mixture
3. Animal and Marine Origin Ingredients
1. Utilizing Light Microscopy to Identify the Origin of Rendered Animal Products in Animal Feeds (Instructor: Neil Vary)
2. Meat & Bone Meal (Instructor: Neil Vary)
3. Lamb Meal, Swine Meal (Instructor: Neil Vary)
4. Poultry By-Product Meal (Instructor: Neil Vary)
5. Blood Meal (Instructor: Neil Vary)
6. Fish Meal (Instructor: Neil Vary)
7. Unknown Mixture
8. Identification of Oilseeds
9. Soybean (Instructor: Neil Vary)
1. Whole
2. Meal
3. Hulls
10. Canola or Rape (Instructor: Neil Vary)
1. Whole
2. Pellets
11. Sunflower (Instructor: Patricia Ramsey)
1. Whole
2. Sunflower seed meal
12. Cottonseed (Instructor: Patricia Ramsey)
1. Whole
2. Meal
3. Hulls
13. Identification of Fiber
14. Alfalfa meal (Instructor: Neil Vary)
15. Beet pulp (Instructor: Patricia Ramsey)
16. Identification of Miscellaneous Products
17. Dried Bakery Product (Instructor: Patricia Ramsey)
18. Unknown Mixture
4. Sample Separation by Flotation (Instructors: Patricia Ramsey, Neil Vary)

Flotation of samples to separate inorganic from organic constituents of feeds & ingredients, this method may applied before or after particle size reduction.

Individual class members will practice flotation upon:
Unknown Ruminant Feed with: 10%, 5%, 3%, 1%, 0.5%, 0.3%, 0.1% Restricted Use Proteins, IE: Meat & bone Meal, Lamb Meal, Swine Meal

1. Micro Chemical Spot Tests (Instructor: Patricia Ramsey, Neil Vary)
1. Silver Nitrate
2. Chloride
3. Phosphates
4. Sulfates
5. Millon Reagent
6. Alizarin Red
7. Bone
8. Iodine Reagent
9. Muscle
2. Unknown Utilizing Bone
3. Final Unknown from Total Products